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ha usp10  (Addgene inc)


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    Addgene inc ha usp10
    Ha Usp10, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ha+usp10/Flag-HA-USP10+(Plasmid+%2322543)/pm40972933-54-0-17
    Average 93 stars, based on 17 article reviews
    ha usp10 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Expressing:

    Article Title: RNF168 and USP10 regulate topoisomerase IIα function via opposing effects on its ubiquitylation
    Article Snippet: IB analyses were performed with anti-Ub (1:2,000; FK2; Biomol international), anti-TOP2α (NeoMarkers), and horseradish peroxidase-conjugated antibodies to mouse (1:3,000, Cell Signaling; 7076) and an enhanced chemiluminescence system (ECL, Amersham). .. Calcium phosphate was used to transfect HEK293T cells with expression vectors for mouse RNF168 (WT and C21S mutant), HA-tagged WT or mutated mouse Rnf168, HA-RNF8, Flag-TOP2α, Flag-TOP2α−κR, HA-USP10 and WT or mutated HA-Ub (Addgene: pRK5-HA-Ub-WT (ID 17608), pRK5-HA-Ub-K48 [ID 17605), pRK5-HA-Ub-K63 (ID 17606)). ..

    Mutagenesis:

    Article Title: RNF168 and USP10 regulate topoisomerase IIα function via opposing effects on its ubiquitylation
    Article Snippet: IB analyses were performed with anti-Ub (1:2,000; FK2; Biomol international), anti-TOP2α (NeoMarkers), and horseradish peroxidase-conjugated antibodies to mouse (1:3,000, Cell Signaling; 7076) and an enhanced chemiluminescence system (ECL, Amersham). .. Calcium phosphate was used to transfect HEK293T cells with expression vectors for mouse RNF168 (WT and C21S mutant), HA-tagged WT or mutated mouse Rnf168, HA-RNF8, Flag-TOP2α, Flag-TOP2α−κR, HA-USP10 and WT or mutated HA-Ub (Addgene: pRK5-HA-Ub-WT (ID 17608), pRK5-HA-Ub-K48 [ID 17605), pRK5-HA-Ub-K63 (ID 17606)). ..



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    USP10 binds to PD-L1 and mediates its deubiquitination. A Identification of the number of candidate proteins interacting with NDR1 and PD-L1 in PC3 cells through CO-IP-MS analysis. B Exogenous PD-L1 coimmunoprecipitated with USP10 in 293 T cells. C USP10 was coimmunoprecipitated with PD-L1 in PC3 cells. D IF experiment was conducted to determine the spatial localization of USP10 and PD-L1. E USP10 expression in prostate cancer cells. F Detection of PD-L1 expression level in PC3 cells after concentration gradient increase of USP10 by WB. G Detection of PD-L1 expression in DU145 cells with siUSP10 gene knockdown by WB. H PD-L1 expression levels in DU145 cells were assessed using a concentration gradient of the USP10 inhibitor (Spautin-1) via immunoblot analysis. I PC3/pCMV and PC3/USP10 cells were subjected with CHX (100 μg/mL) at specified intervals to detect PD-L1-degrading protein levels. J The impact of USP10 on PD-L1 ubiquitination in 293 T cells was detected

    Journal: Cell Communication and Signaling : CCS

    Article Title: NDR1 mediates PD-L1 deubiquitination to promote prostate cancer immune escape via USP10

    doi: 10.1186/s12964-024-01805-5

    Figure Lengend Snippet: USP10 binds to PD-L1 and mediates its deubiquitination. A Identification of the number of candidate proteins interacting with NDR1 and PD-L1 in PC3 cells through CO-IP-MS analysis. B Exogenous PD-L1 coimmunoprecipitated with USP10 in 293 T cells. C USP10 was coimmunoprecipitated with PD-L1 in PC3 cells. D IF experiment was conducted to determine the spatial localization of USP10 and PD-L1. E USP10 expression in prostate cancer cells. F Detection of PD-L1 expression level in PC3 cells after concentration gradient increase of USP10 by WB. G Detection of PD-L1 expression in DU145 cells with siUSP10 gene knockdown by WB. H PD-L1 expression levels in DU145 cells were assessed using a concentration gradient of the USP10 inhibitor (Spautin-1) via immunoblot analysis. I PC3/pCMV and PC3/USP10 cells were subjected with CHX (100 μg/mL) at specified intervals to detect PD-L1-degrading protein levels. J The impact of USP10 on PD-L1 ubiquitination in 293 T cells was detected

    Article Snippet: Plasmids were purchased from Sino Biological: pCMV3-STK38-Myc (HG12319-NM), pCMV3-PD-L1-His (HG10084-CH), pCMV3-USP10-HA (HG17833-CY).

    Techniques: Co-Immunoprecipitation Assay, Expressing, Concentration Assay, Knockdown, Western Blot

    NDR1 promotes binding of USP10 and PD-L1 and inhibits PD-L1 ubiquitination degradation. A Exogenous NDR1 coimmunoprecipitated with USP10 in 293 T cells. B USP10 was coimmunoprecipitated with NDR1 in PC3 cells. C IF experiment revealed the spatial localization of USP10 and NDR1. D Sequential IP assay for NDR1-USP10-PD-L1 interaction. E - F NDR1 knockdown in DU145 cells was assessed for its impact on the protein and mRNA expression of NDR1 and USP10. G-H co-IP assays and IF analysis to explore the relationship between USP10 and PD-L1 in 293T cells, with or without NDR1 overexpression. I: Intranuclear USP10 and PD-L1 expression levels in PC3 cells following a concentration gradient increase of NDR1. J The ubiquitination of intranuclear PD-L1 was examined in 293T cell by WB. K The expression of USP10,NDR1and PD-L1 was detected in NDR1 knockdown or overexpressed DU145 cells with or without Transfected USP10

    Journal: Cell Communication and Signaling : CCS

    Article Title: NDR1 mediates PD-L1 deubiquitination to promote prostate cancer immune escape via USP10

    doi: 10.1186/s12964-024-01805-5

    Figure Lengend Snippet: NDR1 promotes binding of USP10 and PD-L1 and inhibits PD-L1 ubiquitination degradation. A Exogenous NDR1 coimmunoprecipitated with USP10 in 293 T cells. B USP10 was coimmunoprecipitated with NDR1 in PC3 cells. C IF experiment revealed the spatial localization of USP10 and NDR1. D Sequential IP assay for NDR1-USP10-PD-L1 interaction. E - F NDR1 knockdown in DU145 cells was assessed for its impact on the protein and mRNA expression of NDR1 and USP10. G-H co-IP assays and IF analysis to explore the relationship between USP10 and PD-L1 in 293T cells, with or without NDR1 overexpression. I: Intranuclear USP10 and PD-L1 expression levels in PC3 cells following a concentration gradient increase of NDR1. J The ubiquitination of intranuclear PD-L1 was examined in 293T cell by WB. K The expression of USP10,NDR1and PD-L1 was detected in NDR1 knockdown or overexpressed DU145 cells with or without Transfected USP10

    Article Snippet: Plasmids were purchased from Sino Biological: pCMV3-STK38-Myc (HG12319-NM), pCMV3-PD-L1-His (HG10084-CH), pCMV3-USP10-HA (HG17833-CY).

    Techniques: Binding Assay, Knockdown, Expressing, Co-Immunoprecipitation Assay, Over Expression, Concentration Assay, Transfection